Rosy

Joshi-Mukherjee

School of Pharmacy

Education

• BSc, Horticulture, University of Agricultural Sciences, Bangalore, India
• MSc, Horticulture, GB Pant University of Agriculture & Technology, Pantnagar, India
• PhD, Pharmacology, SUNY Upstate Medical University/Syracuse, Advisor: Dr. Mario Delmar
• Postdoctoral Fellow, Department of Biomedical Engineering at Johns Hopkins University/Baltimore, Mentors: Drs. Leslie Tung and David Yue
• Postdoctoral Fellow, Division of Cardiology, School of Medicine at Johns Hopkins University/Baltimore Mentor: Dr. Gordon Tomaselli

Research Interests

• To develop heart-on-a-chip model for electrical measurements, pharmacology, and drug-induced cardiotoxicity studies (Edwards et al., 2018; Zlochiver et. al., 2019).
• To characterize the role of connexin43 a gap junction protein, in human induced pluripotent stem cell reprogramming and differentiation

Publications

1. Zlochiver V, Kroboth SL, Beal CR, Cook JA, *Joshi-Mukherjee R. Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings. J. Vis. Exp. 2019 Jul 15; (149) *corresponding author
2. Edwards SL, Zlochiver V, Conrad DB, Vaidyanathan R, Valiquette AM, *Joshi-Mukherjee R. A Multiwell Cardiac μGMEA Platform for Action Potential Recordings from Human iPSC-Derived Cardiomyocyte Constructs. Stem Cell Reports. 2018;11(2):522-536; *corresponding author.
3. *Joshi-Mukherjee R. Heart-on-a-Chip Model for Cardiomyopathies. Int J Stem Cell Res Transplant. 2017;5 (1e):1-3.
4. Dick, IE; Joshi-Mukherjee, R; Yang, W; and Yue, DT. Arrhythmogenesis in Timothy Syndrome is associated with defects in Ca²⁺ dependent inactivation. Nature Communication. 2016;29;7:10370. Note: conducted in-vitro cell culture experiments, edited and proofread the manuscript.
5. Bazzazi H, Sang L, Dick IE, Joshi-Mukherjee R, Yang W, Yue DT. Novel fluorescence resonance energy transfer-based reporter reveals differential calcineurin activation in neonatal and adult cardiomyocytes. J Physiol. 2015;593(17):3865-84; [OR]. Note: Provided adult cardiomyocytes, mentored the student and proofread the manuscript.
6. Ben-Johny M, Dick IE, Sang L, Limpitikul WB, Kang PW, Niu J, Banerjee R, Yang W, Babich JS, Issa JB, Lee SR, Namkung H, Li J, Zhang M, Yang PS, Bazzazi H, Adams PJ, Joshi-Mukherjee R, Yue DN, Yue DT. Towards a Unified Theory of Calmodulin Regulation (Calmodulation) of Voltage-Gated Calcium and Sodium Channels. Curr Mol Pharmacol. 2015;8(2):188-205. Note: Reviewed and proofread the article
7. Ben-Johny M, Yang PS, Niu J, Yang W, Joshi-Mukherjee R, Yue DT. Conservation of Ca2+/calmodulin regulation across Na and Ca2+ channels. Cell. 2014;157(7):1657-70. Note: Reviewed and proofread the article.
8. Limpitikul WB, Dick IE, Joshi-Mukherjee R, Overgaard MT, George AL Jr, Yue DT. Calmodulin mutations associated with long QT syndrome prevent inactivation of cardiac L-type Ca(2+) currents and promote proarrhythmic behavior in ventricular myocytes. J Mol Cell Cardiol. 2014;74: 115-24. Note: Provided adult cardiomyocytes, mentored the student, reviewed and proofread the manuscript.
9. Joshi-Mukherjee R, Dick I, Ting L, O'Rourke B, Yue DT and Tung L. Structural and Functional Plasticity in Long-term Cultures of Adult Ventricular Myocytes. Journal of Molecular and Cellular Cardiology. 2013;65:76-87. Note: Designed the study, conducted experiments, analyzed the data and wrote the manuscript.
10. Joshi-Mukherjee R, Coombs W, Musa H, Oxford E, Taffet S, Delmar M. Characterization of the molecular phenotype of two arrhythmogenic right ventricular cardiomyopathy (ARVC)-related plakophilin-2 (PKP2) mutations. Heart Rhythm. 2008;5(12):1715-23. Note: Designed the study, conducted experiments, analyzed the data and wrote the manuscript.
11. Joshi-Mukherjee R, Coombs W, Burrer C, Alvarez de Mora I, Delmar M, Taffet, S. Evidence for the presence of a free C-terminal fragment of Cx43 in cultured cells. Cell Communication and Adhesion. 2007;14(2-3):75-84. Note: Designed the study, conducted experiments, analyzed the data and wrote the manuscript.
12. Lewandowski R, Shibayama J, Oxford EM, Joshi-Mukherjee R, Coombs W, Sorgen PL, Taffet SM, Delmar M. Chemical Gating of Connexin Channels. Connexins: A Guide. 2007. Eds. Harris, A.L. and Locke D. Springer, Secaucus, NJ; [BC]. Note: Wrote a section in the chapter and proofread the chapter.

Professional Affiliations & Memberships

• Editorial Activities 
• Journal peer review activities  
• 2014-present     PLOS One
• 2016-present     Circulation: Arrhythmia and Electrophysiology
• 2017-present     Stem Cell Reviews and Reports
• Professional Societies
• 2009-present     AHA, Professional Member
• 2009-present     Biophysical society member
• 2017-present     International Society of Stem Cell Research (ISSCR)
• 2021- Present   American Association of Colleges of Pharmacy (AACP)

Awards, Honors

• 2019 - Travel Award by Harvard Bioscience to present a poster “Reversible Cardiac Action Potential Recordings to Track Cellular Electrophysiology Over Days” at AHA, November 16-18 in Philadelphia, PA
• 2019  - Abstract entitled, Reversible Cardiac Action Potential Recordings to Track Cellular Electrophysiology Over Days, is among the top 10 percent and has been selected to re-present as a poster presentation at Session Title: Best of AHA Specialty Conferences: BCVS 2019 AHA, November 16-18 in Philadelphia, PA
• 2015-2016 - 5 T32 HL 7227-39, Ruth L. Kirschstein Institutional National Research Service Award. Cardiovascular Training GranT, Johns Hopkins Medical University Role: Post-Doctoral Scholar
• 2007 - Travel Award, International Gap Junction Conference