BSc, Horticulture, University of Agricultural Sciences, Bangalore, India
MSc, Horticulture, GB Pant University of Agriculture & Technology, Pantnagar, India
PhD, Pharmacology, SUNY Upstate Medical University/Syracuse, Advisor: Dr. Mario Delmar
Postdoctoral Fellow, Department of Biomedical Engineering at Johns Hopkins University/Baltimore, Mentors: Drs. Leslie Tung and David Yue
Postdoctoral Fellow, Division of Cardiology, School of Medicine at Johns Hopkins University/Baltimore Mentor: Dr. Gordon Tomaselli
To develop heart-on-a-chip model for electrical measurements, pharmacology, and drug-induced cardiotoxicity studies (Edwards et al., 2018; Zlochiver et. al., 2019).
To characterize the role of connexin43 a gap junction protein, in human induced pluripotent stem cell reprogramming and differentiation
Zlochiver V, Kroboth SL, Beal CR, Cook JA, *Joshi-Mukherjee R. Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings. J. Vis. Exp. 2019 Jul 15; (149) *corresponding author
Edwards SL, Zlochiver V, Conrad DB, Vaidyanathan R, Valiquette AM, *Joshi-Mukherjee R. A Multiwell Cardiac μGMEA Platform for Action Potential Recordings from Human iPSC-Derived Cardiomyocyte Constructs. Stem Cell Reports. 2018;11(2):522-536; *corresponding author.
*Joshi-Mukherjee R. Heart-on-a-Chip Model for Cardiomyopathies. Int J Stem Cell Res Transplant. 2017;5 (1e):1-3.
Dick, IE; Joshi-Mukherjee, R; Yang, W; and Yue, DT. Arrhythmogenesis in Timothy Syndrome is associated with defects in Ca2+ dependent inactivation. Nature Communication. 2016;29;7:10370. Note: conducted in-vitro cell culture experiments, edited and proofread the manuscript.
Bazzazi H, Sang L, Dick IE, Joshi-Mukherjee R, Yang W, Yue DT. Novel fluorescence resonance energy transfer-based reporter reveals differential calcineurin activation in neonatal and adult cardiomyocytes. J Physiol. 2015;593(17):3865-84; [OR]. Note: Provided adult cardiomyocytes, mentored the student and proofread the manuscript.
Ben-Johny M, Dick IE, Sang L, Limpitikul WB, Kang PW, Niu J, Banerjee R, Yang W, Babich JS, Issa JB, Lee SR, Namkung H, Li J, Zhang M, Yang PS, Bazzazi H, Adams PJ, Joshi-Mukherjee R, Yue DN, Yue DT. Towards a Unified Theory of Calmodulin Regulation (Calmodulation) of Voltage-Gated Calcium and Sodium Channels. Curr Mol Pharmacol. 2015;8(2):188-205. Note: Reviewed and proofread the article
Ben-Johny M, Yang PS, Niu J, Yang W, Joshi-Mukherjee R, Yue DT. Conservation of Ca2+/calmodulin regulation across Na and Ca2+ channels. Cell. 2014;157(7):1657-70. Note: Reviewed and proofread the article.
Limpitikul WB, Dick IE, Joshi-Mukherjee R, Overgaard MT, George AL Jr, Yue DT. Calmodulin mutations associated with long QT syndrome prevent inactivation of cardiac L-type Ca(2+) currents and promote proarrhythmic behavior in ventricular myocytes. J Mol Cell Cardiol. 2014;74: 115-24. Note: Provided adult cardiomyocytes, mentored the student, reviewed and proofread the manuscript.
Joshi-Mukherjee R, Dick I, Ting L, O'Rourke B, Yue DT and Tung L. Structural and Functional Plasticity in Long-term Cultures of Adult Ventricular Myocytes. Journal of Molecular and Cellular Cardiology. 2013;65:76-87. Note: Designed the study, conducted experiments, analyzed the data and wrote the manuscript.
Joshi-Mukherjee R, Coombs W, Musa H, Oxford E, Taffet S, Delmar M. Characterization of the molecular phenotype of two arrhythmogenic right ventricular cardiomyopathy (ARVC)-related plakophilin-2 (PKP2) mutations. Heart Rhythm. 2008;5(12):1715-23. Note: Designed the study, conducted experiments, analyzed the data and wrote the manuscript.
Joshi-Mukherjee R, Coombs W, Burrer C, Alvarez de Mora I, Delmar M, Taffet, S. Evidence for the presence of a free C-terminal fragment of Cx43 in cultured cells. Cell Communication and Adhesion. 2007;14(2-3):75-84. Note: Designed the study, conducted experiments, analyzed the data and wrote the manuscript.
Lewandowski R, Shibayama J, Oxford EM, Joshi-Mukherjee R, Coombs W, Sorgen PL, Taffet SM, Delmar M. Chemical Gating of Connexin Channels. Connexins: A Guide. 2007. Eds. Harris, A.L. and Locke D. Springer, Secaucus, NJ; [BC]. Note: Wrote a section in the chapter and proofread the chapter.
Professional Affiliations & Memberships
Journal peer review activities
2014-present PLOS One
2016-present Circulation: Arrhythmia and Electrophysiology
2017-present Stem Cell Reviews and Reports
2009-present AHA, Professional Member
2009-present Biophysical society member
2017-present International Society of Stem Cell Research (ISSCR)
2021- Present American Association of Colleges of Pharmacy (AACP)
2019 - Travel Award by Harvard Bioscience to present a poster “Reversible Cardiac Action Potential Recordings to Track Cellular Electrophysiology Over Days” at AHA, November 16-18 in Philadelphia, PA
2019 - Abstract entitled, Reversible Cardiac Action Potential Recordings to Track Cellular Electrophysiology Over Days, is among the top 10 percent and hasbeen selected to re-present as a poster presentation at Session Title: Best of AHA Specialty Conferences: BCVS 2019 AHA, November 16-18 in Philadelphia, PA
2015-2016 - 5 T32 HL 7227-39, Ruth L. Kirschstein Institutional National Research Service Award. Cardiovascular Training GranT, Johns Hopkins Medical University Role: Post-Doctoral Scholar
2007 - Travel Award, International Gap Junction Conference